Western blot abreview for Anti-DDDDK tag antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Li-cor 800
Host species: Monkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
odyssey
Negative control
mock
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 70 kDa Non-specific: 130, 60, 55, 40, 35 kDa
Positive control
transient transfect

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Submitted Apr 22 2016

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