Western blot abreview for Anti-DDDDK tag antibody

Average
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (neuroblastoma2 a cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Specification
neuroblastoma2 a cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2%milk
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Detection

Detection method
ECL+
Negative control
Non-transfection group
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 75 kDa Non-specific: 20, 37, 45, 70, 80 kDa
Positive control
Flag-Senp1 as a positive control, band was observed at 75kDa

Additional data

Additional Notes
For detection of SUMOylation of 5-HT1A receptors, N-Ethylmaleimide (NEM) is used when harvesting cells.

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Submitted Mar 29 2017

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