Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purification notes
    ECS (Enterokinase Cleavage Site) Affinity Purified.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 20371678
ELISA Use at an assay dependent concentration.
Use at a concentration of 2 - 10 µg/ml for coating or 0.03 - 1 µg/ml as a primary.
WB Use at an assay dependent concentration.
Use at a concentration of 0.1 - 1 µg/ml (colorimetric) or 0.03 - 1µg/ml (chemiluminescence).
ICC Use a concentration of 0.5 - 5 µg/ml.

Target

  • Relevance
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Images

  • ab21536 staining exogenous murine FLICE inhibitory protein (FLIP) tagged with FLAG from mouse thyroid tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with BSA prior to incubation with the antibody for 16 hours. A biotinylated goat polyclonal antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:
See all 16 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Merci de nous avoir contactés.

Nous avons plusieurs anti-FLAG anticorps au catalogue par contre aucun n’a été testé en microscopie électronique.

Cependant, je peux vous proposer :

ab1162 (www.abcam.com/ab1162)
ab 18230 (www.abcam.com/ab18230)
ab21536 (www.abcam.com/ab21536)

Ces anticorps n’ont pas encore été testés en microscopie électronique et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester l’un de ces anticorps en microscopie électronique, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, un avoir de la valeur de l'anticorps testé vous sera offert.

Étapes à suivre :

1. Nous informer que vous souhaitez tester un de nos anticorps en microscopie électronique et nous vous enverrons un code promotionnel (valable 4 mois) à utiliser après obtention et envoi de vos résultats,

2. Acheter l'anticorps,

3. Nous envoyer vos résultats (positifs ou négatifs) obtenus sous forme d'Abreview (www.abcam.com/abreviews).

4. L'envoi de vos résultats activera le code et vous donnera un avoir de la valeur de l'anticorps testé.

J'espère que ces informations vous aideront. N'hésitez pas à me contacter si vous avez besoin de plus de renseignements.

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Loading amount
20 µg
Specification
HEK293T
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Ms. Silje Markussen

Verified customer

Submitted Aug 16 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (thyroid)
Specification
thyroid
Fixative
Formaldehyde
Antigen retrieval step
None
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%

Dr. Yujiang FANG

Verified customer

Submitted Mar 29 2007

Answer

Thank you for your enquiry. Jen is out of the office, so I am answering her correspondence. I understand that you used a third antibody successfully in this protocol, but each antibody is different and sometimes must be optimized to work well. What is the specific problem you are getting with each antibody? Are you seeing no signal whatsoever, high background, etc? What blocking solution did you use? 5% milk? BSA? How long and at what temperature did you incubate with the primary antibody? The protocol you included said 2-3 hours at room temperature or overnight at 4 degrees. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. This antibody should recognize both N- terminus or C- terminus DDDDK tag. If you have any additional questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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