Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab122902)

Overview

  • Product name

    Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma)
    See all DDDDK tag primary antibodies
  • Description

    Rabbit polyclonal to DDDDK tag - (Equivalent to FLAG antibodies from Sigma)
  • Host species

    Rabbit
  • Specificity

    ab12902 recognizes Flag-tagged fusion proteins.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    DYKDDDDK

  • Positive control

    • Lysate of 293T cells transfected with the plasmid carrying the Flag-tagged PRMT6 gene.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000.
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Images

  • All lanes : Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab122902) at 1/2000 dilution

    Lane 1 : Lysate of 293T cells transfected with an empty vector
    Lane 2 : Lysate of 293T cells transfected with the plasmid carrying
    the Flag-tagged PRMT6 gene

References

ab122902 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
30 µg
Treatment
transfected with plasmid expressing FLAG
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Sep 08 2018

Answer

This is a good question and difficult to answer. Antibodies to fusion protein tags usually detect without problems no matter where the tag is. However, you would be less likely to encounter any problems if the tag is on the N or C terminus rather than in the middle of the fusion protein sequence. It very much depends on whether the specific fusion protein you have is obscuring the antibody epitope. This would be difficult to know as it is different for each individual case.

I am sorry it is not possible to provide a more definite answer on this occasion. But I hope it is helpful. If you have any further questions, please do not hesitate to contact me.

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