Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab122902)

Overview

  • Product name

    Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma)
    See all DDDDK tag primary antibodies
  • Description

    Rabbit polyclonal to DDDDK tag - (Equivalent to FLAG antibodies from Sigma)
  • Host species

    Rabbit
  • Specificity

    ab12902 recognizes Flag-tagged fusion proteins.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    DYKDDDDK

  • Positive control

    • Lysate of 293T cells transfected with the plasmid carrying the Flag-tagged PRMT6 gene.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000.
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Images

  • All lanes : Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab122902) at 1/2000 dilution

    Lane 1 : Lysate of 293T cells transfected with an empty vector
    Lane 2 : Lysate of 293T cells transfected with the plasmid carrying
    the Flag-tagged PRMT6 gene

References

ab122902 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
30 µg
Treatment
transfected with plasmid expressing FLAG
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

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Verified customer

Submitted Sep 08 2018

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