cell transfected with plasmid without FLAG tagged protein
Specific: none kDa Non-specific: 70, 35 kDa
cell transfected with plasmid expressing FLAG tagged fusion protein
I did western blot on the same lysate with Sigma FLAG antibody (mouse) and worked beautifully. Of course for that I used an anti-mouse secondary antibody. But the bottomline is, this FLAG antibody from abcam clearly does not work.
The reviewer has used this antibody in a tested species and application. The poor performance in this case may be due to lot-to-lot variation. We have offered a refund or replacement as per our Abpromise guarantee.
Abcam user community
Submitted Sep 08 2018
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