Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (FITC) (ab2492)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (FITC)
See all DDDDK tag primary antibodies -
DescriptionRabbit polyclonal to DDDDK tag - (Equivalent to FLAG antibodies from Sigma) (FITC)
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Host speciesRabbit
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ConjugationFITC. Ex: 493nm, Em: 528nm
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Tested applicationsSuitable for: WBmore details
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Immunogen
Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.
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General notesEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
The molar F/P ratio is 4.1.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.1% Sodium azide
Constituent: 0.2% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab2492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Application notes
Prior to conjugation the antibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope™ R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope.
Optimal working dilutions should be determined for your particular assay conditions.
Optimal working dilutions should be determined for your particular assay conditions.
Target
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RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
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Alternative names
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
Protocols
References
This product has been referenced in:
- Inoue N et al. Sema7A/PlxnCl signaling triggers activity-dependent olfactory synapse formation. Nat Commun 9:1842 (2018). Read more (PubMed: 29743476) »
- Guo Q et al. TCN, an AKT inhibitor, exhibits potent antitumor activity and enhances radiosensitivity in hypoxic esophageal squamous cell carcinoma in vitro and in vivo. Oncol Lett 13:949-954 (2017). Read more (PubMed: 28356983) »
