Immunocytochemistry/ Immunofluorescence abreview for Anti-DDDDK tag antibody [F-tag-01]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa, cervical carcinoma)
Specification
HeLa, cervical carcinoma
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,3% Tween20
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Other product details

Dilution
1/200
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS

Secondary antibody

Dilution
1/200

Additional data

PubMed ID
19097142: View
Additional Notes
HeLa cells were transfected with FpCI-Rad51 (Rad51 gene, cloned into FpCI plasmid vector carrying flag tag sequence) and treated with 1uM MMC.
This antibody works great with immunofluorescence applications and Western blot protocols as well.

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Submitted Mar 25 2009

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