Western blot abreview for Anti-DDDDK tag antibody [F-tag-01]

Excellent
Abreviews
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (chimeric protein)
Loading amount
2 µg
Specification
chimeric protein
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS Tween 0.05%

Secondary antibody

Name
Non-Abcam antibody was used: sheep anti mouse hrp conjugate
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 45 kDa

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Submitted Nov 17 2010

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