Immunocytochemistry/ Immunofluorescence abreview for Anti-DDDDK tag antibody [F-tag-01]

Below Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Monkey Cell (COS-7)
Specification
COS-7
Permeabilization
Yes - 0.2% triton
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5%BSA
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
non-specific staining (see image: cos7 cells transfected with a construct has both v5 and flag tag, v5 is showing red, flag is green)
Abcam response
Thank you for submitting your Abreview. We are very sorry to hear that the antibody did not perform as expected in this tested species and application. We are in touch with the customer to resolve this problem according to our Abpromise guarantee (https://www.abcam.com/abpromise) and to the customer’s satisfaction.

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Verified customer

Submitted Sep 12 2013

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