Human Purified protein (FLAG tagged purified SPOCK2 protein)
Gel Running Conditions
FLAG tagged purified SPOCK2 protein
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
2 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: same as blocking buffer
Non-Abcam antibody was used: ECL anti mouse IgG HRP linked while antibody Host species: Sheep Clonality: Polyclonal Conjugation: Horse Radish Peroxidase
With FLAG tagged purified proteins, negative control is not needed. Only 1 band on the blot per lane is possible.
Specific: 50 kDa
The exact same protein and the exact same amount was loaded, but probed with rabbit anti-FLAG from Sigma on a distinct nitrocellulose filter. 1:1000 dilution was used for both primaries.
This antibody clearly works for a western blot, although affinity appears not as strong as the rabbit anti-FLAG from Sigma. To make the uploaded photograph, the two lanes probed with distinct antibodies was spliced to be adjacent in Photoshop. It is possible the difference in band intensity is due to the unique secondary antibodies that had to be used for each primary, since the primaries were made in different species.
Abcam user community
Submitted Nov 19 2018
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