Western blot abreview for Anti-DDDDK tag antibody [F-tag-01]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 transfected with a 27 kDa construct)
Loading amount
25 µg
Specification
HEK293 transfected with a 27 kDa construct
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Stabilized Goat-anti-Mouse HRP-conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 27 kDa Non-specific: 40, 48, 50, 52, 70, 76 kDa
Positive control
No
Negative control
lysate of untransfected cells

Additional data

Additional Notes
The primary antibody was incubated overnight at 4°C. The exact loading amount is not known, because the concentration of protein in the lysate was not measured.

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Submitted Apr 18 2007

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