Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma) (ab125243)

Overview

  • Product name

    Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma)
    See all DDDDK tag primary antibodies
  • Description

    Mouse monoclonal [FG4R] to DDDDK tag - (Equivalent to FLAG antibodies from Sigma)
  • Host species

    Mouse
  • Specificity

    Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.
  • Tested applications

    Suitable for: WB, ELISA, Dot blot, IP, In situ hybridizationmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    DYKDDDDK

  • Positive control

    • HEK293 cells transfected with DYKDDDDK
  • General notes

    If stored at 4 °C please add 0.1% sodium azide.

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab125243 was purified by protein A affinity chromatography from TCS
  • Clonality

    Monoclonal
  • Clone number

    FG4R
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. (with ECL)
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
In situ hybridization 1/500 - 1/2000.

Target

  • Relevance

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Images

  • All lanes : Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma) (ab125243) at 1/1000 dilution

    Lane 1 : HEK293 cell lysate non-transfected
    Lane 2 : HEK293 cell lysate transfected with DDDDK tagged protein vector

    Developed using the ECL technique.

References

This product has been referenced in:

  • Barbarani G  et al. SOX6 blocks the proliferation of BCR-ABL1+ and JAK2V617F+ leukemic cells. Sci Rep 9:3388 (2019). Read more (PubMed: 30833651) »
  • Li D  et al. Armadillo repeat containing 12 promotes neuroblastoma progression through interaction with retinoblastoma binding protein 4. Nat Commun 9:2829 (2018). Read more (PubMed: 30026490) »
See all 4 Publications for this product

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