Western blot abreview for Anti-DDDDK tag antibody [M2] (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Saccharomyces cerevisiae Purified protein (BJ5453)
Loading amount
1 µg
Specification
BJ5453
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBST+5%Milk

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
3 second(s)
Bands
Specific: 81 kDa

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Submitted Jan 14 2009

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