Western blot abreview for Anti-DDDDK tag antibody [M2] (HRP)

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Abreviews
Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Loading amount
1 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBST+5%Milk

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 77 kDa

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Submitted Apr 27 2010

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