Western blot abreview for Anti-DDDDK tag antibody [M2] (HRP)

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Abreviews
Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (CHO-S, transfected with plasmid containing insert)
Loading amount
125000 cells
Specification
CHO-S, transfected with plasmid containing insert
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Other product details

Dilution
1/5000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 37 kDa Non-specific: 28 kDa
Positive control
CHO-S, transfected with plasmid containing insert with FLAG-tagged protein of interest

Additional data

Additional Notes
PVDF membrane used , lysed with Pierce M-Per, Pierce Halt protease and phosphatase inhibitors used.

Lane 1: Bio-rad Precision Plus Kaleidoscope Markers (hand-drawn on)
Lane 2: Blank
Lane 3: Lysate 25000 cells (CHO-S, transfected with plasmid containing insert with FLAG-tagged protein of interest)
Lane 4: Blank
Lane 5: Lysate 625000 cells (CHO-S, transfected with plasmid containing insert with FLAG-tagged protein of interest).
Lane 6: Blank
Lane 7: Lysate 125000 cells (CHO-S, transfected with plasmid containing insert with FLAG-tagged protein of interest)

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Submitted Jun 15 2010

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