Western blot abreview for Anti-DDDDK tag antibody [M2] (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (chimeric protein)
Loading amount
2 µg
Specification
chimeric protein
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS tween 0.05%

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 41 kDa

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Submitted Nov 17 2010

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