Western blot abreview for Anti-DDDDK tag antibody [M2] (HRP)

Good
Abreviews
Application
Western blot
Sample
Human Purified protein (NIH3T3 cells)
Loading amount
5e+006 cells
Specification
NIH3T3 cells
Treatment
10 mM Sodium Butyrate for 24 hrs, 500 ug/ml Geneticin for 48 hrs
Gel Running Conditions
Reduced Denaturing (3-8 % Tris-Acetate Gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 200 kDa Non-specific: 31, 117, 71 kDa
Positive control
Used the positive CTL from the IP kit we used

Additional data

Additional Notes
This antibody worked great. The issue was our samples and getting our flag tagged gene to express the signal. It took some time to optimize the antibody to our samples, but it worked fine when we did.

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Submitted Jan 07 2011

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