Overview

  • Product name
    Anti-DDDDK tag antibody [M2] (SureLight® Allophycocyanin)
    See all DDDDK tag primary antibodies
  • Description
    Mouse monoclonal [M2] to DDDDK tag - (Equivalent to FLAG antibodies from Sigma) (SureLight® Allophycocyanin)
  • Host species
    Mouse
  • Conjugation
    SureLight® Allophycocyanin. Ex: 652nm, Em: 657nm
  • Tested applications
    Suitable for: FRET, Flow Cytmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Synthetic peptide: DYKDDDDK.

  • General notes

    This antibody has been optimized for High throughput screening (HTS) and fluorescence resonance energy transfer (FRET) assays.

    Excitation maximum:652 nm Emission maximum:657.5 nm F:P 1:1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    pH: 7.40
    Preservative: 0.013% Sodium azide
    Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose
  • Concentration information loading...
  • Purity
    Size exclusion
  • Purification notes
    Purified using size exclusion chromatography as well as some proprietary methods for purifying the final materials.
  • Primary antibody notes
    This antibody has been optimized for High throughput screening (HTS) and fluorescence resonance energy transfer (FRET) assays.
  • Clonality
    Monoclonal
  • Clone number
    M2
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FRET Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

References

This product has been referenced in:
  • Roy G  et al. A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies. MAbs 10:416-430 (2018). Read more (PubMed: 29400603) »
  • Hayashi Y  et al. Carboxyl-terminal Tail-mediated Homodimerizations of Sphingomyelin Synthases Are Responsible for Efficient Export from the Endoplasmic Reticulum. J Biol Chem 292:1122-1141 (2017). Read more (PubMed: 27927984) »
See all 4 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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