Product nameAnti-DDDDK tag (Binds to FLAG® tag sequence) antibody
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies
DescriptionChicken polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
Species reactivityReacts with: Species independent
Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were affinity purified using the peptide coupled to a different carrier.
Our Abpromise guarantee covers the use of ab1170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/5000 - 1/20000.|
|WB||1/2000 - 1/10000.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
ab1170 staining DDDDK in Mouse primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 1x PBS + 5% Goat serum + 0.1% Triton-X 100) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-chicken IgY polyclonal (1/400) was used as the secondary antibody.
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170) at 1/5000 dilution
Rabbit Anti-Chicken IgY H&L (HRP) (ab6753) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 66 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
This product has been referenced in:
- Lim J et al. Nuclear Proximity of Mtr4 to RNA Exosome Restricts DNA Mutational Asymmetry. Cell 169:523-537.e15 (2017). Read more (PubMed: 28431250) »
- Hutson MR et al. Temperature-activated ion channels in neural crest cells confer maternal fever-associated birth defects. Sci Signal 10:N/A (2017). Read more (PubMed: 29018170) »