Product nameAnti-DDDDK tag (Binds to FLAG® tag sequence) antibody
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies
DescriptionGoat polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
Species reactivityReacts with: Species independent
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab1257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/100 - 1/500.|
|WB||1/1000 - 1/30000.|
|ICC/IF||1/200 - 1/2000.|
RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
Human microvascular endothelial cells expressing DDDDK-tagged beta-catenin following transient transfection.
Ab1257 used at 1 µg/ml. Detected with FITC labeled rabbit anti-goat IgG (H&L) secondary antibody.
All lanes : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1257) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate. at 0.2 µg
Lane 2 : E. coli whole cell lysate. at 0.1 µg
Lane 3 : E. coli whole cell lysate. at 0.05 µg
Western Blot analysis of E. coli Whole Cell Lysates, labelled with DDDDK-tagged Protein by ab1257 at 1/25,000.
This product has been referenced in:
- Xu W et al. Endothelial FAM3A positively regulates post-ischaemic angiogenesis. EBioMedicine 43:32-42 (2019). Read more (PubMed: 31000420) »
- Park HS et al. NS1 Protein of 2009 Pandemic Influenza A Virus Inhibits Porcine NLRP3 Inflammasome-Mediated Interleukin-1 Beta Production by Suppressing ASC Ubiquitination. J Virol 92:N/A (2018). Read more (PubMed: 29386291) »