Product nameAnti-DDDDK tag (Binds to FLAG® tag sequence) antibody
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies
DescriptionRabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
Tested applicationsSuitable for: IHC-P, ELISA, WB, ICCmore details
Species reactivityReacts with: Species independent
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
Purification notesECS (Enterokinase Cleavage Site) Affinity Purified.
Our Abpromise guarantee covers the use of ab21536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 20371678|
|ELISA||Use at an assay dependent concentration.
Use at a concentration of 2 - 10 µg/ml for coating or 0.03 - 1 µg/ml as a primary.
|WB||Use at an assay dependent concentration.
Use at a concentration of 0.1 - 1 µg/ml (colorimetric) or 0.03 - 1µg/ml (chemiluminescence).
|ICC||Use a concentration of 0.5 - 5 µg/ml.|
RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
ab21536 staining exogenous murine FLICE inhibitory protein (FLIP) tagged with FLAG® from mouse thyroid tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with BSA prior to incubation with the antibody for 16 hours. A biotinylated goat polyclonal antibody was used as the secondary.
This product has been referenced in:
- Czarna A et al. Single-cell analysis of the fate of c-kit-positive bone marrow cells. NPJ Regen Med 2:27 (2017). Read more (PubMed: 29302361) »
- Urasaki Y et al. Chronic Uridine Administration Induces Fatty Liver and Pre-Diabetic Conditions in Mice. PLoS One 11:e0146994 (2016). WB . Read more (PubMed: 26789264) »