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I attached the protocol that I used. Actually, My VSVG antibody (catalog number:ab18612-200) that we ordered together with those two antibodiies worked when I used this protocol. Thank you so much.
Asked on Dec 08 2005
Thank you for your enquiry. Jen is out of the office, so I am answering her correspondence. I understand that you used a third antibody successfully in this protocol, but each antibody is different and sometimes must be optimized to work well. What is the specific problem you are getting with each antibody? Are you seeing no signal whatsoever, high background, etc? What blocking solution did you use? 5% milk? BSA? How long and at what temperature did you incubate with the primary antibody? The protocol you included said 2-3 hours at room temperature or overnight at 4 degrees. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.
Answered on Dec 08 2005