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I'd like to do Immunoprecipitation by using ab1233 and ab1240. Is that including agarose bead and antibodies?
Then, when I use that product, how could I do "pre-clear step"?
It is my first time to do IP, and as I known "pre-clear"step is needed for reducing non-specific pull down.
Please let me know if it is not necessary.
Asked on Mar 06 2012
Thank you for your inquiry.
Pre-clearing the lysates is always up to the end-user to determine because background levels will vary. Since both of these antibodies are conjugated to agarose, if you want to pre-clear the lysates you could use an isotype control.
For your information, we do have a suitable isotype control for both ab1233 and ab1240 as a goat IgG Agarose conjugated antibody, ab104155.
Also, here is our general IP protocol:
I hope this information helps. Please contact us with any other questions.
Answered on Mar 06 2012