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I am going to use this product Anti-DDDDK tag antibody (Agarose) (ab1240) and I was wondering if you have any guidelines to use these beads, especially at which speed can I centrifuge them and for how long? How many times to wash the beads and which buffer to use for this purpose? Thanks a lot!
Asked on Nov 01 2012
Thank you for contacting Abcam.
You would use the product, ab1240 as you would use regular Protein A/G Sepharose beads, same type of buffers and centrifugation steps. I have attached the Abcam protocols booklet, which contains a general IP protocol that you would be able to use for guidance.
If there is anything else I can help you with, please let me know.
Answered on Nov 01 2012