Immunocytochemistry/ Immunofluorescence abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa, cervical carcinoma)
Permeabilization
Yes - 0,3% Tween20
Specification
HeLa, cervical carcinoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/200

Secondary antibody

Dilution
1/200

Additional data

Additional Notes
HeLa cells were transfected with FpCI-Rad51 (Rad51 gene, cloned into FpCI plasmid vector carrying flag tag sequence) and treated with 1uM MMC. This antibody works great with immunofluorescence applications and Western blot protocols as well.
PubMed ID
19097142: View

Abcam user community

Verified customer

Submitted Mar 25 2009

Sign up