Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293 cells transfected with empty vector or FH-FLAG)
Gel Running Conditions
Reduced Denaturing (Pierce precise protein gel 4-20%)
Loading amount
20 µg
Specification
293 cells transfected with empty vector or FH-FLAG
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3.0% Non-fat milk, PBS, 0.1% Tween-20
Concentration
2 µg/ml

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti mouse-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Supersignal West Dura
Negative control
Cells transfected with empty vector
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 55 kDa
Positive control
Cells transfected with FH-FLAG

Additional data

Additional Notes
lane 1: 293 transfected with empty vector lane 2: 293 transfected with FH-FLAG (~50kDa)

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Submitted Dec 17 2010

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