Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01]

Good
Abreviews
Application
Western blot
Sample
Mouse Recombinant protein (Rear leg muscle (homogenised))
Gel Running Conditions
Reduced Denaturing (16% Tricine)
Loading amount
20 µg
Specification
Rear leg muscle (homogenised)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS Tween 0.05%
Dilution
1/1000

Secondary antibody

Dilution
1/4000
Name
Non-Abcam antibody was used: anti mse hrp conjugate
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 4 kDa

Additional data

Additional Notes
Expected band at 4 kDa observed.

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Submitted Mar 04 2011

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