Product nameAnti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies
DescriptionMouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence)
Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged (also known as the FLAG® tag sequence) fusion proteins.
Tested applicationsSuitable for: WB, ELISA, Dot blot, IP, In situ hybridizationmore details
Species reactivityReacts with: Species independent
- HEK293 cells transfected with DYKDDDDK (also known as the FLAG® tag sequence).
If stored at 4°C please add 0.1% sodium azide.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Purification notesab125243 was purified by protein A affinity chromatography from TCS
Our Abpromise guarantee covers the use of ab125243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/3000. (with ECL)|
|ELISA||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|In situ hybridization||1/500 - 1/2000.|
RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
This product has been referenced in:
- Barbarani G et al. SOX6 blocks the proliferation of BCR-ABL1+ and JAK2V617F+ leukemic cells. Sci Rep 9:3388 (2019). Read more (PubMed: 30833651) »
- Li D et al. Armadillo repeat containing 12 promotes neuroblastoma progression through interaction with retinoblastoma binding protein 4. Nat Commun 9:2829 (2018). Read more (PubMed: 30026490) »