Product nameAnti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (Biotin)
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies
DescriptionMouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence) (Biotin)
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Species independent
- E. coli lysate containing a multi-epitope tagged protein.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab173832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000.|
|IP||Use at an assay dependent concentration.|
RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
All lanes : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (Biotin) (ab173832) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg
All lanes : Streptavidin-HRP at 1/20000 dilution
Developed using the ECL technique.
Note: Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Incubation with ab173832 was for 1 hour at room temperature. Incubation with secondary antibody was for 30 miutes at room temperature.
ab173832 has not yet been referenced specifically in any publications.