• Product name

    Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (FITC)
    See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies
  • Description

    Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence) (FITC)
  • Host species

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.

  • General notes

    Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

    The molar F/P ratio is 4.1.

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab2492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    Prior to conjugation the antibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope™ R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope.
    Optimal working dilutions should be determined for your particular assay conditions.
  • Target

    • Relevance

      This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
    • Alternative names

      • DDDDK epitope tag antibody
      • DDDK antibody
      • ddk antibody
      • DYKDDDDK antibody
      • DYKDDDDK epitope tag antibody
      • DYKDDDDK tag antibody
      • ECS epitope tag antibody
      • ECS tag antibody
      • Enterokinase Cleavage Site epitope tag antibody
      • Enterokinase Cleavage Site tag antibody
      • FLAG® antibody
      • FLAG® tag antibody
      see all


    This product has been referenced in:

    • Inoue N  et al. Sema7A/PlxnCl signaling triggers activity-dependent olfactory synapse formation. Nat Commun 9:1842 (2018). Read more (PubMed: 29743476) »
    • Guo Q  et al. TCN, an AKT inhibitor, exhibits potent antitumor activity and enhances radiosensitivity in hypoxic esophageal squamous cell carcinoma in vitro and in vivo. Oncol Lett 13:949-954 (2017). Read more (PubMed: 28356983) »
    See all 2 Publications for this product

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