Anti-DDHD2 antibody (ab235832)
Key features and details
- Rabbit polyclonal to DDHD2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDHD2 antibody -
Description
Rabbit polyclonal to DDHD2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human DDHD2 aa 350-650.
Database link: O94830 -
Positive control
- WB: K562 and U-251 MG whole cell lysates. IHC-P: Human small intestine tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Hydrolysis
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 81 kDa.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 81 kDa. |
Target
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Function
Phospholipase that hydrolyzes preferentially phosphatidic acid and phosphatidylethanolamine. May be involved in the maintenance of the endoplasmic reticulum and/or Golgi structures. -
Tissue specificity
Ubiquitously expressed (at protein level). -
Sequence similarities
Belongs to the PA-PLA1 family.
Contains 1 DDHD domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 WWE domain. -
Cellular localization
Cytoplasm. Cytoplasmic granule. - Information by UniProt
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Database links
- Entrez Gene: 23259 Human
- SwissProt: O94830 Human
- Unigene: 434966 Human
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Alternative names
- DDHD domain containing protein 2 antibody
- DDHD domain-containing protein 2 antibody
- Ddhd2 antibody
see all
Images
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All lanes : Anti-DDHD2 antibody (ab235832) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 81 kDa -
Paraffin-embedded human small intestine tissue stained for DDHD2 using ab235832 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235832 has not yet been referenced specifically in any publications.