Key features and details
- Rabbit polyclonal to DDI2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DDI2 antibody
See all DDI2 primary antibodies
DescriptionRabbit polyclonal to DDI2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab197081 was affinity purified using an epitope specific to DDI2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab197081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 45 kDa.|
Sequence similaritiesBelongs to the DDI1 family.
Contains 1 ubiquitin-like domain.
- Information by UniProt
- DDI1 DNA damage inducible 1 homolog 2 antibody
- Ddi2 antibody
- DDI2_HUMAN antibody
All lanes : Anti-DDI2 antibody (ab197081) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
Detection of DDI2 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab197081 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab197081 has been referenced in 1 publication.
- Nowak K et al. Inhibition of calpain-1 stabilizes TCF11/Nrf1 but does not affect its activation in response to proteasome inhibition. Biosci Rep 38:N/A (2018). PubMed: 30177525