Key features and details
- Rabbit polyclonal to DDI2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DDI2 antibody
See all DDI2 primary antibodies
DescriptionRabbit polyclonal to DDI2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human DDI2 aa 1-211.
MLLTVYCVRRDLSEVTFSLQVDADFELHNFRALCELESGIPAAESQIVYA ERPLTDNHRSLASYGLKDGDVVILRQKENADPRPPVQFPNLPRIDFSSIA VPGTSSPRQRQPPGTQQSHSSPGEITSSPQGLDNPALLRDMLLANPHELS LLKERNPPLAEALLSGDLEKFSRVLVEQQQDRARREQERIRLFSADPFDL EAQAKIEEDIR
Database link: Q5TDH0
- WB: A431, HL-60, K562 and Caco-2 whole cell lysates. IHC-P: Human endometrial cancer tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 45 kDa.|
|IHC-P||1/20 - 1/200.|
Sequence similaritiesBelongs to the DDI1 family.
Contains 1 ubiquitin-like domain.
- Information by UniProt
- DDI1 DNA damage inducible 1 homolog 2 antibody
- Ddi2 antibody
- DDI2_HUMAN antibody
All lanes : Anti-DDI2 antibody (ab235791) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 45 kDa
Paraffin-embedded human endometrial cancer tissue stained for DDI2 using ab235791 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235791 has not yet been referenced specifically in any publications.