Recombinant
RabMAb

Recombinant Anti-DDIT3 antibody [EPR4943(2)] - BSA and Azide free (ab240220)

Overview

  • Product name

    Anti-DDIT3 antibody [EPR4943(2)] - BSA and Azide free
    See all DDIT3 primary antibodies
  • Description

    Rabbit monoclonal [EPR4943(2)] to DDIT3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DDIT3 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P35638

  • General notes

    Ab240220 is the carrier-free version of ab179823. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240220 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat. 

 

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.
    • Involvement in disease

      Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS.
    • Sequence similarities

      Belongs to the bZIP family.
      Contains 1 bZIP domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • C/EBP homologous protein antibody
      • C/EBP Homology Protein antibody
      • C/EBP zeta antibody
      • C/EBP-homologous protein 10 antibody
      • C/EBP-homologous protein antibody
      • CCAAT/enhancer binding protein homologous protein antibody
      • CEBPZ antibody
      • CHOP 10 antibody
      • CHOP antibody
      • CHOP-10 antibody
      • CHOP10 antibody
      • DDIT 3 antibody
      • DDIT-3 antibody
      • Ddit3 antibody
      • DDIT3_HUMAN antibody
      • DNA Damage Inducible Transcript 3 antibody
      • DNA damage-inducible transcript 3 protein antibody
      • GADD 153 antibody
      • GADD153 antibody
      • Growth Arrest and DNA Damage Inducible Protein 153 antibody
      • Growth arrest and DNA damage inducible protein GADD153 antibody
      • Growth arrest and DNA damage-inducible protein GADD153 antibody
      • MGC4154 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DDIT3 using ab179823 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179823).

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling DDIT3 using ab179823 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179823).

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    References

    ab240220 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240220.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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