Anti-DDIT3 (phospho S30) antibody (ab63392)
Key features and details
- Rabbit polyclonal to DDIT3 (phospho S30)
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDIT3 (phospho S30) antibody
See all DDIT3 primary antibodies -
Description
Rabbit polyclonal to DDIT3 (phospho S30) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of GADD153 only when phosphorylated at serine 30. -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human DDIT3 (phospho S30).
Database link: P35638 -
Positive control
- A549 cells and human breast carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/500 - 1/1000.
|
|
IHC-P |
1/50 - 1/100.
|
Notes |
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ICC/IF
1/500 - 1/1000. |
IHC-P
1/50 - 1/100. |
Target
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Function
Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. -
Involvement in disease
Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS. -
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1649 Human
- Entrez Gene: 13198 Mouse
- Omim: 126337 Human
- SwissProt: P35638 Human
- SwissProt: P35639 Mouse
- Unigene: 505777 Human
- Unigene: 728989 Human
- Unigene: 110220 Mouse
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Alternative names
- C/EBP homologous protein antibody
- C/EBP Homology Protein antibody
- C/EBP zeta antibody
see all
Images
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Immunofluorescence analysis of A549 cells, using ab63392 at 1:500 dilution.
Left image untreated.Right image treated with phospho peptide. -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab63392 at 1:50 dilution.
Left image untreated.Right image treated with phospho peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab63392 has been referenced in 1 publication.
- Tian H et al. D4F alleviates the C/EBP homologous protein-mediated apoptosis in glycated high-density lipoprotein-treated macrophages by facilitating autophagy. Exp Biol Med (Maywood) 246:2595-2609 (2021). PubMed: 34525858