Overview

  • Product name

    Anti-DDR2 antibody [3B11E4]
    See all DDR2 primary antibodies
  • Description

    Mouse monoclonal [3B11E4] to DDR2
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WB, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human DDR2. Purified truncated recombinant Human DDR2 expressed in E. Coli strain BL21 (DE3)

  • Positive control

    • ICC/IF: A549 cells Flow Cyt: A549 cells Gc-7901 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab63337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/200.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/2000. Predicted molecular weight: 97 kDa.
ELISA 1/10000.
ICC/IF 1/500 - 1/2000.
IHC-P 1/500 - 1/2000.

Target

  • Function

    This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing.
  • Tissue specificity

    The major 10 kDa transcript is expressed in high levels in heart and lung, less in brain, placenta, liver, skeletal muscle, pancreas, and kidney.
  • Involvement in disease

    Defects in DDR2 are the cause of spondyloepimetaphyseal dysplasia short limb-hand type (SEMD-SL) [MIM:271665]. A bone disease characterized by short-limbed dwarfism, a narrow chest with pectus excavatum, brachydactyly in the hands and feet, a characteristic craniofacial appearance and premature calcifications. The radiological findings are distinctive and comprise short long bones throughout the skeleton with striking epiphyses that are stippled, flattened and fragmented and flared, irregular metaphyses. Platyspondyly in the spine with wide intervertebral spaces is observed and some vertebral bodies are pear-shaped with central humps, anterior protrusions and posterior scalloping.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 1 F5/8 type C domain.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD167 antigen-like family member B antibody
    • CD167b antibody
    • CD167b antigen antibody
    • Cell migration inducing protein 20 antibody
    • DDR 2 antibody
    • DDR2 antibody
    • DDR2_HUMAN antibody
    • Discoidin domain containing receptor 2 antibody
    • Discoidin domain receptor 2 antibody
    • Discoidin domain receptor family member 2 antibody
    • discoidin domain receptor tyrosine kinase 2 antibody
    • Discoidin domain-containing receptor 2 antibody
    • discoidin domain-containing receptor tyrosine kinase 2 antibody
    • Hydroxyaryl protein kinase antibody
    • MIG20a antibody
    • Migration inducing gene 16 protein antibody
    • Neurotrophic tyrosine kinase antibody
    • Neurotrophic tyrosine kinase receptor related 3 antibody
    • NTRKR 3 antibody
    • NTRKR3 antibody
    • Receptor protein tyrosine kinase TKT antibody
    • Receptor protein-tyrosine kinase TKT antibody
    • Receptor related 3 antibody
    • receptor-related 3 antibody
    • TKT antibody
    • TYRO 10 antibody
    • TYRO10 antibody
    • Tyrosine kinase receptor related to neurotrophic TRK antibody
    • Tyrosine protein kinase TYRO 10 antibody
    • Tyrosine protein kinase TYRO10 antibody
    • Tyrosine-protein kinase TYRO10 antibody
    • Tyrosylprotein kinase antibody
    see all

Images

  • All lanes : Anti-DDR2 antibody [3B11E4] (ab63337) at 1/500 dilution

    Lane 1 : molecular weight markers
    Lane 2 : truncated recombinant DDR2 (immunizing peptide)

    Predicted band size: 97 kDa

  • ab63337 at 1/1000 dilution staining DDR2 in human skeletal muscle tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections).

  • ab63337 at 1/1000 dilution staining DDR2 in human A549 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® conjugated Goat anti-mouse was used as secondary. The actin filaments were labeled red with DY-554 phalloidin whilst nuclei were stained blue with DRAQ5 fluorescent DNA dye.

  • Overlay histogram showing A549 cells stained with ab63337 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63337, 1/200 dilution) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) preadsorbed (ab150117) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a (ab170191, 0.5μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References

This product has been referenced in:

  • Skiöldebrand E  et al. Inflammatory activation of human cardiac fibroblasts leads to altered calcium signaling, decreased connexin 43 expression and increased glutamate secretion. Heliyon 3:e00406 (2017). Read more (PubMed: 29022008) »
  • Suutre S  et al. Evaluation of correlation of articular cartilage staining for DDR2 and proteoglycans with histological tissue damage and the results of radiographic assessment in patients with early stages of knee osteoarthritis. Int J Clin Exp Pathol 8:5658-65 (2015). IHC . Read more (PubMed: 26191278) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Sample
Human Cell (iPS-derived cardiofibroblast)
Specification
iPS-derived cardiofibroblast
Permeabilization
Yes - 0.2% triton X
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 05 2014

Answer

Vielen Dank für Ihre Frage.

Leider wurde die Konzentration des ab63337 nicht bestimmt. Dieser Antikörper ist ein Ascite und wurde nicht purifiziert. Normalerweise haben Asciten circa 1 bis 10mg/ml totale Antikörper . Dieser Antikörper wurde in dieser Form in den auf dem Datenblatt angebenen Verdünnungen getestet.

Ich hoffe, diese Information ist Ihnen hilfreich. Bitte zögern Sie nicht uns wieder zu kontaktieren, sollten Sie weitere Fragen oder Bedenken haben.

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Answer

Thank you for your patience. I had to wait forconfirmation on theimmunogen and the flow cytometry testing procedure with the lab.

I can confirm that the immunogen is mainly intracellular but also contains part of a trans-mebrane domain.

Our lab alsoa flow cytometry protocol that uses fixation without permeabilisation with this antibodyand hadgood results.

I would like to re-assure you that this antibody is covered by our Abpromise guarantee.

If you would like to use fixation and permeabilisation I do not think that should be a problem. We would like to encourage you to submit an Abreview about your experiments with this antibody. We value feedback form our customers highly and you could earn Abpoints with Abreview that can be redeemed as discount on future purchases or Amazon gift vouchers.

I am sorry I could not provide a definitive answer on this occasion and hope this information is nevertheless helpful. Please do not hesitate to contact us again with any further questions.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jan 27 2012

Question
Answer

Anbei wie versprochen die Publikationen aus der Gruppe von Gabbiani bzw. Bochaton-Piallat (PMIDs 21868702 bzw. 17347479). Ich hoffe, diese helfen Ihnen zumindest als Ausgangspunkt für Ihre weitere Suche nach geeigneten Markern. Vielleicht ist einer von beiden ja auch zu einer Kooperation bereit? (Meine Kollegin hat beide in sehr netter Erinnerung). Ihren Abreview haben wir gerade erhalten und ich habe ihn veröffentlicht. Damit ist der Gutscheincode freigeschalten und Sie können sich einen kostenlosen primären Antikörper Ihrer Wahl bei uns bestellen. Falls Sie sich dafür entscheiden, den DDR2-Antikörper ab63337 zu testen, sende ich Ihnen wie besprochen den neuen Rabattcode. Das humane DDR2 hat laut BLAST eine 97%ige Sequenzähnlichkeit mit dem DDR2 des Schweins (SwissProt ID F1S212, http://www.uniprot.org/uniprot/F1S212). DISCOUNT CODE: 100%ABR-T7T0Q Ablaufdatum: 24.04.2012 Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer 100%ABR-T7T0Q und kann zum Kauf weiterer Produkte eingesetzt werden. Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test im Schwein eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer. Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind. Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount Ich wünsche Ihnen ein frohes Fest und alles Gute im Neuen Jahr sowie viel Glück und Erfolg für Ihre Forschung!

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