Overview

  • Product name
  • Description
    Rabbit polyclonal to DDR2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide CYFRSEASEWEPNAISF conjugated to KLH, corresponding to amino acids 304-320 of Human DDR2.

  • Positive control
    • HL60 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 102 kDa).

Target

  • Function
    This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing.
  • Tissue specificity
    The major 10 kDa transcript is expressed in high levels in heart and lung, less in brain, placenta, liver, skeletal muscle, pancreas, and kidney.
  • Involvement in disease
    Defects in DDR2 are the cause of spondyloepimetaphyseal dysplasia short limb-hand type (SEMD-SL) [MIM:271665]. A bone disease characterized by short-limbed dwarfism, a narrow chest with pectus excavatum, brachydactyly in the hands and feet, a characteristic craniofacial appearance and premature calcifications. The radiological findings are distinctive and comprise short long bones throughout the skeleton with striking epiphyses that are stippled, flattened and fragmented and flared, irregular metaphyses. Platyspondyly in the spine with wide intervertebral spaces is observed and some vertebral bodies are pear-shaped with central humps, anterior protrusions and posterior scalloping.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 1 F5/8 type C domain.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD167 antigen-like family member B antibody
    • CD167b antibody
    • CD167b antigen antibody
    • Cell migration inducing protein 20 antibody
    • DDR 2 antibody
    • DDR2 antibody
    • DDR2_HUMAN antibody
    • Discoidin domain containing receptor 2 antibody
    • Discoidin domain receptor 2 antibody
    • Discoidin domain receptor family member 2 antibody
    • discoidin domain receptor tyrosine kinase 2 antibody
    • Discoidin domain-containing receptor 2 antibody
    • discoidin domain-containing receptor tyrosine kinase 2 antibody
    • Hydroxyaryl protein kinase antibody
    • MIG20a antibody
    • Migration inducing gene 16 protein antibody
    • Neurotrophic tyrosine kinase antibody
    • Neurotrophic tyrosine kinase receptor related 3 antibody
    • NTRKR 3 antibody
    • NTRKR3 antibody
    • Receptor protein tyrosine kinase TKT antibody
    • Receptor protein-tyrosine kinase TKT antibody
    • Receptor related 3 antibody
    • receptor-related 3 antibody
    • TKT antibody
    • TYRO 10 antibody
    • TYRO10 antibody
    • Tyrosine kinase receptor related to neurotrophic TRK antibody
    • Tyrosine protein kinase TYRO 10 antibody
    • Tyrosine protein kinase TYRO10 antibody
    • Tyrosine-protein kinase TYRO10 antibody
    • Tyrosylprotein kinase antibody
    see all

Images

  • ab5520 at a 1/100 dilution staining approximately 97kDa band of TYRO 10 in  HL60 cell lysate by Western blot (ECL). ab5520 at a 1/100 dilution staining approximately 97kDa band of TYRO 10 in HL60 cell lysate by Western blot (ECL).

References

This product has been referenced in:
  • Li T  et al. Incorporation of DDR2 clusters into collagen matrix via integrin-dependent posterior remnant tethering. Int J Biol Sci 14:654-666 (2018). Read more (PubMed: 29904280) »
  • Allodi I  et al. Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS. Sci Rep 6:25960 (2016). Read more (PubMed: 27180807) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question

Thank you for your reply. We'll send claim sheet to you soon. Please confirm the attached file and give me the answer. ............................... Please include test protocol information. In a detail, please. 1. H460 Cells were washed with PBS and lysed in 1x Lamelli buffer. 2. The total cell lysate was loaded in 7% SDS-PAGE gel and run at 50V. 3. Protein was transferred to PVDF and the membrane was washed briefly with TBS, then incubated in blocking buffer (TBS, 5% skim milk) overnight at 4oC 4. The membrane was then incubated with anti-rabbit polyclonal antibody for DDR2 (1:500 dilution, abcam) in the blocking buffer for 5 hours at 4oC. 5. After being washed five times with TBS, the membrane was incubated with horseradish peroxidase-conjugated secondary antibody (1:2000 for anti-rabbit, SantaCruz) for 2hours at room temperature. 6. After being washed five times with TBS, the corresponding protein band was visualized using chemiluminescence detection kit(Amersham). --------------------------------------------------------------- With our previous batch of DDR2 antibody from abcam that we bought last year, we had been quite successful in western blotting for DDR2. However when we changed the DDR2 antibody with a new batch (Lot number; 78867) that we bought last May 25, suddenly we could not detect any specific DDR2 protein band and we keep having problems even though we did not change the experimental protocol and used the same cell lysate. Therefore we think the DDR2 antibody we purchased recently could have some problems.

Read More
Answer

Thank you for your e-mail, we had difficulty opening this attachement and I needed the help for our IT department, can you please not send your claim sheets as attached files but copy them and paste them in an e-mail? Can you please provide me with: 1) the order number and lot number of the vial that worked for the researcher so that I can compare those and see if the problem is batch dependent? 2) To arrange for a replacement I will need the order number of the vial of lot 78867, can you please send this information too? My apologies for the delay, your cooperation is appreciated,

Read More

Answer

Thank you for your enquiry I am sorry to hear that you have been experiencing problems with this product. From the information provided it is difficult to offer specific advice, as the information is too sparse. However from the data that you have provided me with I do have a few suggestions to make and questions that will help me to understand your protocol. 1) Sample= "cell extract". What type of cell extract are using? What species is the cell extract derived from? Are you using a specific sub cellular fraction? 2) The primary has been used at a dilution factor of 1:500? What other dilutions have you used the Ab at, as it should be optimised according to the results gained at 1:500. 4) I would suggest you use a more concentrated dilution factor. you have not provided information regarding the period of time that you incubate with the prim. and secondary Ab for but this may also need to be optimised and increased. 5) The B-actin Ab is a loading control, what results did you get for this? 6) You should use a control that is known to be positive for the target. The suggested positive control for this product is HL60 cell lysate. A positive control is essential in order to indicate whether the results you are obtaining are due to Ab /protocol or that the target is not present in the samples that you are using. 7) What steps have you taken when repeating the experiment in order to optimise the conditions? Good luck with your research. If after implementing these suggestions you are still having trouble using this product then please get back in touch with me. I look forward to hearing from you.

Read More

Answer

Thank you for your patience. I forwarded the details of your protocol and your results found to the originator of ab5520. They predict that the 95 kDa band is DDR2. The appearance of a single additional band with a difference of +10 kDa could suggest a posttranslational modification such as sumoylation or ubiquitination. They checked the DDR2 sequence which revealed some predicted sumoylation sites. The peptide was not glycosylated. If you have any more questions or comments, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up