Key features and details
- Rabbit polyclonal to DDT
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DDT antibody
See all DDT primary antibodies
DescriptionRabbit polyclonal to DDT
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to a sequence at the C-terminal of Human DDT.
- Human placenta tissue; Rat brain, pancreas, liver and lung tissue lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab115785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 13 kDa.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionTautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).
Sequence similaritiesBelongs to the MIF family.
- Information by UniProt
- D dopachrome decarboxylase antibody
- D dopachrome tautomerase antibody
- D-dopachrome decarboxylase antibody
DDT was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab115785 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex DAB as the chromogen.
All lanes : Anti-DDT antibody (ab115785) at 0.5 µg/ml
Lane 1 : rat liver tissues lysate
Lane 2 : rat testis tissues lysate
Lanes 3-4 : mouse liver tissues lysate
Lysates/proteins at 50 µg per lane.
All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 13 kDa
All lanes : Anti-DDT antibody (ab115785) at 1 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat pancreas tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Rat lung tissue lysate
Predicted band size: 13 kDa
ab115785 at 1µg/ml staining DTT in Paraffin-embedded Human placenta tissue by Immunohistochemistry.
ab115785 has not yet been referenced specifically in any publications.