Overview

  • Product name

  • Description

    Rabbit polyclonal to DDT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a sequence at the C-terminal of Human DDT.

  • Positive control

    • Human placenta tissue; Rat brain, pancreas, liver and lung tissue lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 13 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Images

  • DDT was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab115785 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex DAB as the chromogen.

  • All lanes : Anti-DDT antibody (ab115785) at 0.5 µg/ml

    Lane 1 : rat liver tissues lysate
    Lane 2 : rat testis tissues lysate
    Lanes 3-4 : mouse liver tissues lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution

    Predicted band size: 13 kDa

  • All lanes : Anti-DDT antibody (ab115785) at 1 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat pancreas tissue lysate
    Lane 3 : Rat liver tissue lysate
    Lane 4 : Rat lung tissue lysate

    Predicted band size: 13 kDa

  • ICC/IF image of ab115785 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115785 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab115785 at 1µg/ml staining DTT in Paraffin-embedded Human placenta tissue by Immunohistochemistry.

References

ab115785 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - other (rat or mouse heart soluble fraction)
Loading amount
100 µg
Specification
rat or mouse heart soluble fraction
Gel Running Conditions
Reduced Denaturing (18%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jan 17 2013

Question
Answer

Thank you for your inquiry.

I heard back from the lab that they used the C-terminal sequence of DDT as the immunogen. There is no cross-reactivity expected with mouse MIF according to a BLAST alignment.


To our knowledge, ab115785 has not been tested in mouse. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

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Answer

free of charge replacement arranged.

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Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.
We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.
If the product is to be used in an untested species or application, you are eligible for our testing discount program if the antibody has not yet been purchased.
To our knowledge, ab115785 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab115785 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of ab115785 off a future order.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab115785 in mouse. I will then send a discount code. This code must be issued before purchasing ab115785 so please wait for my reply before ordering.
2. Purchase ab115785 either by phone, fax, or online (www.abcam.com).
3. Test it in mouse.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab115785 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Application
Immunoprecipitation
Sample
Mouse Tissue lysate - other (liver cytosol)
Total protein in input
500 µg
Specification
liver cytosol
Immuno-precipitation step
Other - Dynabeads bound to Protein A

Abcam user community

Verified customer

Submitted Mar 02 2012

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