Western blot abreview for Anti-DDT antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (rat or mouse heart soluble fraction)
Loading amount
100 µg
Specification
rat or mouse heart soluble fraction
Gel Running Conditions
Reduced Denaturing (18%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Concentration
1 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Diluent: TBST

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 13 kDa
Positive control
rat sample

Additional data

Additional Notes
Lane 1: 0.5 ug recombinant mouse MIF
Lane 2: rat heart cytosolic fraction, 100 ug
Lane 3: wild type mouse heart cytosolic fraction, 100 ug
Lane 4: MIF KO mouse heart cytosolic fraction, 100 ug
The samples were run on an 18 % PAGE gel.

ABTRIAL-TBD5I mouse

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Submitted Jan 17 2013

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