Key features and details
- Rabbit polyclonal to DDT
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DDT antibody
See all DDT primary antibodies
DescriptionRabbit polyclonal to DDT
SpecificityNo cross reactivity with other proteins.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 13 kDa.|
FunctionTautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).
Sequence similaritiesBelongs to the MIF family.
- Information by UniProt
- D dopachrome decarboxylase antibody
- D dopachrome tautomerase antibody
- D-dopachrome decarboxylase antibody
DDT was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab191050 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Anti-DDT antibody (ab191050) at 0.5 µg/ml + Recombinant Human DDT Protein at 0.5 µg
goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 13 kDa
ab191050 has not yet been referenced specifically in any publications.