Key features and details
- Rabbit polyclonal to DDX1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DDX1 antibody
See all DDX1 primary antibodies
DescriptionRabbit polyclonal to DDX1
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 650 and the C-terminus (residue 697) of human DDX1. (AAH12739.1)
- Whole cell lysate from Hela cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 3-5 µg/mg of lysate.|
FunctionActs as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs.
Tissue specificityHighest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX1 subfamily.
Contains 1 B30.2/SPRY domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
DomainThe helicase domain is involved in the stimulation of RELA transcriptional activity.
modificationsPhosphorylated. Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR).
Cellular localizationNucleus. Cytoplasm. Cytoplasmic granule. Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells. Required for proper localization of HIV-1 Rev.
- Information by UniProt
- ATP dependent helicase DDX1 antibody
- ATP-dependent RNA helicase DDX1 antibody
- DBP RB antibody
All lanes : Anti-DDX1 antibody (ab70252) at 0.03 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 20 µg
Lane 2 : Whole cell lysate from Hela cells at 40 µg
Lane 3 : Whole cell lysate from Hela cells at 80 µg
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 50 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling DDX1 with ab70252 at 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation/ Western Blot of DDX1
HeLa whole cell lysate, (1 mg for IP, 1/4 of IP loaded) with ab70252 at 0.03µg/ml for WB.
Lane 1: ab70251 at 3µg/mg lysate for IP.
Lane 2: Control IgG.
Detection: Chemiluminescence with exposure time of 1 minute.
ab70252 has been referenced in 1 publication.
- Wang IX et al. Human proteins that interact with RNA/DNA hybrids. Genome Res 28:1405-1414 (2018). PubMed: 30108179