• Product name

  • Description

    Rabbit polyclonal to DDX11
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human DDX11 aa 1-220.
    Database link: Q96FC9

  • Positive control

    • WB: Mouse gonadal tissue; K562 whole cell lysate. IHC-P: Human tonsil and gastric cancer tissues.



Our Abpromise guarantee covers the use of ab230017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
IHC-P 1/20 - 1/200.


  • Function

    DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitement of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA.
  • Tissue specificity

    Highly expressed in spleen, B-cells, thymus, testis, ovary, small intestine, and pancreas. Very low expression seen in the brain. Expressed in dividing cells and/or cells undergoing high levels of recombination. No expression is seen in cells signaled to terminally differentiate. Expressed in keratinocyte growth factor-stimulated cells but not in serum, EGF and IL1-beta-treated keratinocytes.
  • Involvement in disease

    Defects in DDX11 are the cause of Warsaw breakage syndrome (WBRS) [MIM:613398]. It is a syndrome characterized by severe microcephaly, pre- and postnatal growth retardation, facial dysmorphism and abnormal skin pigmentation. Additional features include high arched palate, coloboma of the right optic disk, deafness, ventricular septal defect, toes and fingers abnormalities. At cellular level, drug-induced chromosomal breakage, a feature of Fanconi anemia, and sister chromatid cohesion defects, a feature of Roberts syndrome, coexist.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily.
    Contains 1 helicase ATP-binding domain.
  • Cellular localization

    Nucleus. Nucleus > nucleolus. During the early stages of mitosis, localizes to condensed chromatin and is released from the chromatin with progression to metaphase. Also localizes to the spindle poles throughout mitosis and at the midbody at later stages of mitosis (metaphase to telophase). Co-localizes with bovine papillomavirus type 1 regulatory protein E2 at early stages of mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • CHL1 antibody
    • CHL1 related helicase gene 1 antibody
    • CHL1-like helicase homolog antibody
    • CHL1-related protein 1 antibody
    • CHLR1 antibody
    • Ddx11 antibody
    • DDX11_HUMAN antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 11 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae) antibody
    • DEAD/H box antibody
    • DEAD/H box protein 11 antibody
    • hCHLR1 antibody
    • Keratinocyte growth factor regulated gene 2 protein antibody
    • Keratinocyte growth factor-regulated gene 2 protein antibody
    • KRG 2 antibody
    • KRG-2 antibody
    • KRG2 antibody
    • MGC133249 antibody
    • MGC9335 antibody
    • OTTHUMP00000238778 antibody
    • OTTHUMP00000238780 antibody
    • OTTHUMP00000238784 antibody
    • OTTHUMP00000238785 antibody
    • Probable ATP dependent RNA helicase DDX11 antibody
    • Probable ATP-dependent RNA helicase DDX11 antibody
    • WABS antibody
    see all


  • Paraffin-embedded human gastric cancer tissue stained for DDX11 using ab230017 at 1/100 dilution in immunohistochemical analysis.

  • Praffin-embedded human tonsil tissue stained for DDX11 using ab230017 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-DDX11 antibody (ab230017) at 1/1000 dilution

    Lane 1 : Mouse gonadal tissue.
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 108 kDa
    Observed band size: 108 kDa


ab230017 has not yet been referenced specifically in any publications.

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