Key features and details
- Rabbit polyclonal to DDX11
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DDX11 antibody
See all DDX11 primary antibodies
DescriptionRabbit polyclonal to DDX11
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human DDX11 aa 1-220.
Database link: Q96FC9
- WB: Mouse gonadal tissue; K562 whole cell lysate. IHC-P: Human tonsil and gastric cancer tissues.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionDNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitement of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA.
Tissue specificityHighly expressed in spleen, B-cells, thymus, testis, ovary, small intestine, and pancreas. Very low expression seen in the brain. Expressed in dividing cells and/or cells undergoing high levels of recombination. No expression is seen in cells signaled to terminally differentiate. Expressed in keratinocyte growth factor-stimulated cells but not in serum, EGF and IL1-beta-treated keratinocytes.
Involvement in diseaseDefects in DDX11 are the cause of Warsaw breakage syndrome (WBRS) [MIM:613398]. It is a syndrome characterized by severe microcephaly, pre- and postnatal growth retardation, facial dysmorphism and abnormal skin pigmentation. Additional features include high arched palate, coloboma of the right optic disk, deafness, ventricular septal defect, toes and fingers abnormalities. At cellular level, drug-induced chromosomal breakage, a feature of Fanconi anemia, and sister chromatid cohesion defects, a feature of Roberts syndrome, coexist.
Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily.
Contains 1 helicase ATP-binding domain.
Cellular localizationNucleus. Nucleus > nucleolus. During the early stages of mitosis, localizes to condensed chromatin and is released from the chromatin with progression to metaphase. Also localizes to the spindle poles throughout mitosis and at the midbody at later stages of mitosis (metaphase to telophase). Co-localizes with bovine papillomavirus type 1 regulatory protein E2 at early stages of mitosis.
- Information by UniProt
- CHL1 antibody
- CHL1 related helicase gene 1 antibody
- CHL1-like helicase homolog antibody
Paraffin-embedded human gastric cancer tissue stained for DDX11 using ab230017 at 1/100 dilution in immunohistochemical analysis.
Praffin-embedded human tonsil tissue stained for DDX11 using ab230017 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-DDX11 antibody (ab230017) at 1/1000 dilution
Lane 1 : Mouse gonadal tissue.
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230017 has not yet been referenced specifically in any publications.