• Product name
  • Description
    Mouse polyclonal to DDX11
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length DDX11 protein (Human)

  • Positive control
    • transfected 293T cell lysate



Our Abpromise guarantee covers the use of ab66971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/100. Detects a band of approximately 125 kDa (predicted molecular weight: 108 kDa).


  • Function
    DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitement of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA.
  • Tissue specificity
    Highly expressed in spleen, B-cells, thymus, testis, ovary, small intestine, and pancreas. Very low expression seen in the brain. Expressed in dividing cells and/or cells undergoing high levels of recombination. No expression is seen in cells signaled to terminally differentiate. Expressed in keratinocyte growth factor-stimulated cells but not in serum, EGF and IL1-beta-treated keratinocytes.
  • Involvement in disease
    Defects in DDX11 are the cause of Warsaw breakage syndrome (WBRS) [MIM:613398]. It is a syndrome characterized by severe microcephaly, pre- and postnatal growth retardation, facial dysmorphism and abnormal skin pigmentation. Additional features include high arched palate, coloboma of the right optic disk, deafness, ventricular septal defect, toes and fingers abnormalities. At cellular level, drug-induced chromosomal breakage, a feature of Fanconi anemia, and sister chromatid cohesion defects, a feature of Roberts syndrome, coexist.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily.
    Contains 1 helicase ATP-binding domain.
  • Cellular localization
    Nucleus. Nucleus > nucleolus. During the early stages of mitosis, localizes to condensed chromatin and is released from the chromatin with progression to metaphase. Also localizes to the spindle poles throughout mitosis and at the midbody at later stages of mitosis (metaphase to telophase). Co-localizes with bovine papillomavirus type 1 regulatory protein E2 at early stages of mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHL1 antibody
    • CHL1 related helicase gene 1 antibody
    • CHL1-like helicase homolog antibody
    • CHL1-related protein 1 antibody
    • CHLR1 antibody
    • Ddx11 antibody
    • DDX11_HUMAN antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 11 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae) antibody
    • DEAD/H box antibody
    • DEAD/H box protein 11 antibody
    • hCHLR1 antibody
    • Keratinocyte growth factor regulated gene 2 protein antibody
    • Keratinocyte growth factor-regulated gene 2 protein antibody
    • KRG 2 antibody
    • KRG-2 antibody
    • KRG2 antibody
    • MGC133249 antibody
    • MGC9335 antibody
    • OTTHUMP00000238778 antibody
    • OTTHUMP00000238780 antibody
    • OTTHUMP00000238784 antibody
    • OTTHUMP00000238785 antibody
    • Probable ATP dependent RNA helicase DDX11 antibody
    • Probable ATP-dependent RNA helicase DDX11 antibody
    • WABS antibody
    see all


  • All lanes : Anti-DDX11 antibody (ab66971) at 1/500 dilution

    Lane 1 : DDX11 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 108 kDa
    Observed band size: 125 kDa (why is the actual band size different from the predicted?)


This product has been referenced in:
  • Calì F  et al. Tim/Timeless, a member of the replication fork protection complex, operates with the Warsaw breakage syndrome DNA helicase DDX11 in the same fork recovery pathway. Nucleic Acids Res 44:705-17 (2016). IP . Read more (PubMed: 26503245) »

See 1 Publication for this product

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