Overview

  • Product name

  • Description

    Rabbit polyclonal to DDX17
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human DDX17.

    Read Abcam's proprietary immunogen policy (Peptide available as ab31640.)

  • Positive control

    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate ; A431 (Human epithelial carcinoma cell line) Whole Cell Lysate; Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate; HEK 293 (Human embryonic kidney cell line) Whole Cell Lysate;

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23223531
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 80,73 kDa (predicted molecular weight: 80,73 kDa).

This antibody was raised against an immunogen that is predicted to cross react with both isoform 1 (80 kDa) and isoform 2 (73 kDa) of Human DDX17(SwissProt data).

Target

  • Function

    RNA-dependent ATPase activity.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ddx17 antibody
    • DDX17_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box helicase 17 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 17 antibody
    • DEAD box helicase 17 antibody
    • DEAD box protein 17 antibody
    • DEAD box protein p72 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 17 antibody
    • P72 antibody
    • Probable ATP-dependent RNA helicase DDX17 antibody
    • RH70 antibody
    • RNA dependent helicase p72 antibody
    • RNA-dependent helicase p72 antibody
    see all

Images

  • All lanes : Anti-DDX17 antibody (ab24601) at 1 µg/ml

    Lane 1 : Wild-type HEK 293 whole cell lysate
    Lane 2 : DDX17 knockout HEK 293 whole cell lysate
    Lane 3 : Hela whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 80,73 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab24601 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab24601 was shown to recognize DDX17 in wild-type HEK 293 cells as signal was lost at the expected MW in DDX17 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. Ab24601 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-DDX17 antibody (ab24601) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 80,73 kDa
    Observed band size: 73,80 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 14 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab24601 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Lambert MP  et al. The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation. Nucleic Acids Res 46:7686-7700 (2018). Read more (PubMed: 29931089) »
  • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). Read more (PubMed: 30377371) »
See all 7 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100

Dr. Kirk Mcmanus

Verified customer

Submitted Sep 16 2011

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