Product nameAnti-DDX17 antibody
See all DDX17 primary antibodies
DescriptionRabbit polyclonal to DDX17
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
- HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate ; A431 (Human epithelial carcinoma cell line) Whole Cell Lysate; Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate; HEK 293 (Human embryonic kidney cell line) Whole Cell Lysate;
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab24601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 23223531|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 80,73 kDa (predicted molecular weight: 80,73 kDa).
This antibody was raised against an immunogen that is predicted to cross react with both isoform 1 (80 kDa) and isoform 2 (73 kDa) of Human DDX17(SwissProt data).
FunctionRNA-dependent ATPase activity.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Ddx17 antibody
- DDX17_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box helicase 17 antibody
All lanes : Anti-DDX17 antibody (ab24601) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80,73 kDa
Observed band size: 73,80 kDa why is the actual band size different from the predicted?
Additional bands at: 14 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab24601 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
This product has been referenced in:
- Le Sage V et al. Proteomic analysis of HIV-1 Gag interacting partners using proximity-dependent biotinylation. Virol J 12:138 (2015). Read more (PubMed: 26362536) »
- Samaan S et al. The Ddx5 and Ddx17 RNA helicases are cornerstones in the complex regulatory array of steroid hormone-signaling pathways. Nucleic Acids Res N/A:N/A (2013). WB ; Human . Read more (PubMed: 24275493) »