Product nameAnti-DDX17 antibody
See all DDX17 primary antibodies
DescriptionRabbit polyclonal to DDX17
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
Synthetic peptide corresponding to a region between C terminal residues 550 and 613 of human DDX17
- Whole cell lysate from HeLa, 293T, MCF7 and K562 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70184 was affinity purified using an epitope specific to DDX17 immobilized on solid support.
Our Abpromise guarantee covers the use of ab70184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/15000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).|
FunctionRNA-dependent ATPase activity.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Ddx17 antibody
- DDX17_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box helicase 17 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DDX17 with ab70184 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-DDX17 antibody (ab70184) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : MCF7 whole cell lysate at 50 µg
Lane 6 : K562 whole cell lysate at 50 µg
Predicted band size: 72 kDa
Observed band size: 72 kDa
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
This product has been referenced in:
- Motiño O et al. Regulation of MicroRNA 183 by Cyclooxygenase 2 in Liver Is DEAD-Box Helicase p68 (DDX5) Dependent: Role in Insulin Signaling. Mol Cell Biol 35:2554-67 (2015). WB . Read more (PubMed: 25963660) »
- Zaccara S et al. p53-directed translational control can shape and expand the universe of p53 target genes. Cell Death Differ N/A:N/A (2014). Read more (PubMed: 24926617) »