Key features and details
- Rabbit polyclonal to DDX17
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DDX17 antibody
See all DDX17 primary antibodies
DescriptionRabbit polyclonal to DDX17
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
Synthetic peptide corresponding to a region between C terminal residues 550 and 613 of human DDX17
- Whole cell lysate from HeLa, 293T, MCF7 and K562 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70184 was affinity purified using an epitope specific to DDX17 immobilized on solid support.
Our Abpromise guarantee covers the use of ab70184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/15000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).|
FunctionRNA-dependent ATPase activity.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Ddx17 antibody
- DDX17_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box helicase 17 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DDX17 with ab70184 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-DDX17 antibody (ab70184) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : MCF7 whole cell lysate at 50 µg
Lane 6 : K562 whole cell lysate at 50 µg
Predicted band size: 72 kDa
Observed band size: 72 kDa
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
ab70184 has been referenced in 3 publications.
- Fu K et al. Biological and RNA regulatory function of MOV10 in mammalian germ cells. BMC Biol 17:39 (2019). PubMed: 31088452
- Motiño O et al. Regulation of MicroRNA 183 by Cyclooxygenase 2 in Liver Is DEAD-Box Helicase p68 (DDX5) Dependent: Role in Insulin Signaling. Mol Cell Biol 35:2554-67 (2015). WB . PubMed: 25963660
- Zaccara S et al. p53-directed translational control can shape and expand the universe of p53 target genes. Cell Death Differ N/A:N/A (2014). PubMed: 24926617