Overview

  • Product name

    Anti-DDX17 antibody [EPR13807(B)] - C-terminal
    See all DDX17 primary antibodies
  • Description

    Rabbit monoclonal [EPR13807(B)] to DDX17 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DDX17 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q92841

  • Positive control

    • 293T, HeLa and MCF7 cell lysates; Human breast tissue; 293T cells; MCF7 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13807(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100 - 1/250.

Antigen retrieval is recommended.

WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      RNA-dependent ATPase activity.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Ddx17 antibody
      • DDX17_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box helicase 17 antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 17 antibody
      • DEAD box helicase 17 antibody
      • DEAD box protein 17 antibody
      • DEAD box protein p72 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 17 antibody
      • P72 antibody
      • Probable ATP-dependent RNA helicase DDX17 antibody
      • RH70 antibody
      • RNA dependent helicase p72 antibody
      • RNA-dependent helicase p72 antibody
      see all

    Images

    • All lanes : Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190) at 1/5000 dilution

      Lane 1 : Wild-type HEK 293 whole cell lysate
      Lane 2 : DDX17 knockout HEK 293 whole cell lysate
      Lane 3 : Hela whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 72 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab180190 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab180190 was shown to recognize DDX17 in wild-type HEK 293 cells as signal was lost at the expected MW in DDX17 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab180190 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 72 kDa

    • ab180190 staining DDX17 antibody in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Negative control 1:PBS only.

    • Immunofluorescent analysis of 293T cells labeling DDX17 with ab180190 at 1/100.

    • Immunohistochemical analysis of paraffin embedded Human breast tissue labeling DDX17 with ab180190 at 1/100.

    • Flow Cytometrical analysis of permeabilized MCF7 cells labeling DDX17 with ab180190 at 1/10 (red) or a rabbit IgG negative (green).

    References

    This product has been referenced in:

    • Geiger R  et al. L-Arginine Modulates T Cell Metabolism and Enhances Survival and Anti-tumor Activity. Cell 167:829-842.e13 (2016). Human . Read more (PubMed: 27745970) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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