Product nameAnti-DDX17 antibody [EPR13807(B)] - C-terminal
See all DDX17 primary antibodies
DescriptionRabbit monoclonal [EPR13807(B)] to DDX17 - C-terminal
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human DDX17 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q92841
- 293T, HeLa and MCF7 cell lysates; Human breast tissue; 293T cells; MCF7 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100 - 1/250.
Antigen retrieval is recommended.
|WB||1/1000 - 1/10000. Predicted molecular weight: 72 kDa.|
FunctionRNA-dependent ATPase activity.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Ddx17 antibody
- DDX17_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box helicase 17 antibody
All lanes : Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190) at 1/5000 dilution
Lane 1 : Wild-type HEK 293 whole cell lysate
Lane 2 : DDX17 knockout HEK 293 whole cell lysate
Lane 3 : Hela whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 72 kDa
Lanes 1 - 4: Merged signal (red and green). Green - ab180190 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab180190 was shown to recognize DDX17 in wild-type HEK 293 cells as signal was lost at the expected MW in DDX17 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab180190 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 72 kDa
ab180190 staining DDX17 antibody in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Negative control 1:PBS only.
Immunofluorescent analysis of 293T cells labeling DDX17 with ab180190 at 1/100.
Immunohistochemical analysis of paraffin embedded Human breast tissue labeling DDX17 with ab180190 at 1/100.
Flow Cytometrical analysis of permeabilized MCF7 cells labeling DDX17 with ab180190 at 1/10 (red) or a rabbit IgG negative (green).