Anti-DDX18 antibody (ab128197)
Key features and details
- Rabbit polyclonal to DDX18
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDX18 antibody
See all DDX18 primary antibodies -
Description
Rabbit polyclonal to DDX18 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified Human DDX18 (Q9NVP1) protein fused with GST
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Positive control
- HeLa whole cell extracts and HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 75 kDa.
|
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| ICC/IF |
1/500.
|
| Notes |
|---|
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WB
1/1000 - 1/2000. Predicted molecular weight: 75 kDa. |
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ICC/IF
1/500. |
Target
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Function
Probable RNA-dependent helicase. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. - Information by UniProt
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Database links
- Entrez Gene: 8886 Human
- Omim: 606355 Human
- SwissProt: Q9NVP1 Human
- Unigene: 728867 Human
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Alternative names
- ATP dependent RNA helicase DDX18 antibody
- ATP-dependent RNA helicase DDX18 antibody
- DDX 18 antibody
see all
Images
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Western blot - Anti-DDX18 antibody (ab128197)Anti-DDX18 antibody (ab128197) at 1/1000 dilution + HeLa whole cell extract at 10 µg
Predicted band size: 75 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-DDX18 antibody (ab128197)ab128197, at 1/500, staing of DDX18 in HeLa cells followed by FITC-conjugated anti-rabbit IgG second antibody by immunohistochemistry. DNA was stained with Hoechst dye.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab128197 has been referenced in 1 publication.
- Wang IX et al. Human proteins that interact with RNA/DNA hybrids. Genome Res 28:1405-1414 (2018). PubMed: 30108179