Anti-DDX18 antibody (ab128197)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-DDX18 antibody
See all DDX18 primary antibodies -
Description
Rabbit polyclonal to DDX18 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Purified Human DDX18 (Q9NVP1) protein fused with GST
-
Positive control
- HeLa whole cell extracts and HeLa cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab128197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Predicted molecular weight: 75 kDa. | |
| ICC/IF | 1/500. |
Target
-
Function
Probable RNA-dependent helicase. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. - Information by UniProt
-
Database links
- Entrez Gene: 8886 Human
- Entrez Gene: 66942 Mouse
- Entrez Gene: 308490 Rat
- Omim: 606355 Human
- SwissProt: Q9NVP1 Human
- SwissProt: Q8K363 Mouse
- Unigene: 728867 Human
- Unigene: 44219 Mouse
-
Alternative names
- ATP dependent RNA helicase DDX18 antibody
- ATP-dependent RNA helicase DDX18 antibody
- DDX 18 antibody
see all
Images
-
Western blot - Anti-DDX18 antibody (ab128197)Anti-DDX18 antibody (ab128197) at 1/1000 dilution + HeLa whole cell extract at 10 µg
Predicted band size: 75 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-DDX18 antibody (ab128197)ab128197, at 1/500, staing of DDX18 in HeLa cells followed by FITC-conjugated anti-rabbit IgG second antibody by immunohistochemistry. DNA was stained with Hoechst dye.
Datasheets and documents
References
This product has been referenced in:
- Wang IX et al. Human proteins that interact with RNA/DNA hybrids. Genome Res 28:1405-1414 (2018). Read more (PubMed: 30108179) »
