Key features and details
- Rabbit polyclonal to DDX18
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DDX18 antibody
See all DDX18 primary antibodies
DescriptionRabbit polyclonal to DDX18
SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Baboon, Orangutan
A region between residue 625 and the C terminus (residue 670) of human DDX18 (NP_006764.3)
- Whole cell lysate from HeLa and 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProbable RNA-dependent helicase.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX18/HAS1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
- Information by UniProt
- ATP dependent RNA helicase DDX18 antibody
- ATP-dependent RNA helicase DDX18 antibody
- DDX 18 antibody
All lanes : Anti-DDX18 antibody (ab70527) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 3 minutes
Detection of DDX18 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70526 (Lane 1) and ab70527 (Lane 2) at 3 µg/mg lysate for IP. ab70527 was used at 1 µg/ml for subsequent WB detection. Lane 3 represents control IgG IP.
Chemiluminescence with an exposure time of 30 seconds.
ab70527 has not yet been referenced specifically in any publications.