Anti-DDX19B antibody (ab151478)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-DDX19B antibody
See all DDX19B primary antibodies -
Description
Rabbit polyclonal to DDX19B -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-218 of Human DDX19B (UniProt: Q9UMR2).
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Positive control
- A549, HeLa, and HepG2 whole cell lysate (ab7900); HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab151478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 54 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
ATP-dependent RNA helicase involved in mRNA export from the nucleus. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Cytoplasm. Nucleus > nuclear pore complex. Nucleus membrane. Nuclear pore complex cytoplasmic fibrils. - Information by UniProt
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Database links
- Entrez Gene: 517438 Cow
- Entrez Gene: 11269 Human
- Entrez Gene: 234733 Mouse
- Entrez Gene: 690693 Rat
- Omim: 605812 Human
- SwissProt: Q3ZBV2 Cow
- SwissProt: Q9UMR2 Human
- Unigene: 221761 Human
see all -
Alternative names
- ATP-dependent RNA helicase DDX19B antibody
- DBP5 antibody
- Dbp5, yeast, homolog of antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DDX19B antibody (ab151478)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling DDX19B with ab151478 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342. -
Western blot - Anti-DDX19B antibody (ab151478)All lanes : Anti-DDX19B antibody (ab151478) at 1/10000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
10% SDS PAGE
References
ab151478 has not yet been referenced specifically in any publications.
